Zinc sulphate improved microspore embryogenesis in barley
نویسندگان
چکیده
منابع مشابه
Expression profiles in barley microspore embryogenesis
In barley, microarray technology has allowed to study the gene expression profiling associated with the stress pretreatment phase of microspore embryogenesis, where the reprogramming of microspores takes place. Transcriptome analysis of anthers before and after four days of mannitol pretreatment, revealed changes in the expression of 2,673 genes. A thorough study of these differentially express...
متن کاملChanges in DNA methylation levels and nuclear distribution patterns after microspore reprogramming to embryogenesis in barley.
Under specific stress treatments, the microspore can be induced in vitro to deviate from its gametophytic development and to reprogram towards embryogenesis, becoming a totipotent cell and forming haploid embryos. These can further regenerate homozygous plants for production of new isogenic lines, an important biotechnological tool for crop breeding. DNA methylation constitutes a prominent epig...
متن کاملThe early microspore embryogenesis pathway in barley is accompanied by concrete ultrastructural and expression changes
*Address correspondence to: María del Carmen Risueño. Plant Development and Nuclear Organization, Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006 Madrid, Spain. e-mail: [email protected] ABSTRACT The use of dihaploid plants for obtaining new varieties has been widely reported in different plant species. The regeneration of these plants is carried out by in vitro induction of ...
متن کاملTranscriptome analysis of barley anthers: effect of mannitol treatment in microspore embryogenesis
Carbohydrate starvation is an efficient stress treatment for induction of microspore embryogenesis. Transcriptome analysis of anthers response to mannitol treatment using the 22k Barley1 GeneChip revealed large changes in gene expression. Statistical analysis and filtering for 4-fold or greater changes resulted in 2,673 genes, of which 887 were up-regulated and 1,786 down-regulated. Great diffe...
متن کامل5-azacytidine promotes microspore embryogenesis initiation by decreasing global DNA methylation, but prevents subsequent embryo development in rapeseed and barley
Microspores are reprogrammed by stress in vitro toward embryogenesis. This process is an important tool in breeding to obtain double-haploid plants. DNA methylation is a major epigenetic modification that changes in differentiation and proliferation. We have shown changes in global DNA methylation during microspore reprogramming. 5-Azacytidine (AzaC) cannot be methylated and leads to DNA hypome...
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ژورنال
عنوان ژورنال: Plant Cell, Tissue and Organ Culture
سال: 2008
ISSN: 0167-6857,1573-5044
DOI: 10.1007/s11240-008-9376-y